Coding
tphB

Part:BBa_K808010:Design

Designed by: Sascha Hein, Sven Rumpf, Daniel Sachs   Group: iGEM12_TU_Darmstadt   (2012-09-02)

tphB: reaction from DCD to protocatechuate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 76

TphB is an terephthalate dihydrodiol dehydrogenase from Comamonas testosteroni and catalyzes the reaction from 4,5-dihydroxy-2,6-cyclohexadiene-1,4-dicarboxylate to protocatechuate.


Design Notes

We have isolated tphB with colony PCR


Source

The genetic donor of tphB is Comamonas testosteroi KF-1

References